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NDV Virulent&Universal PCR

The Detection Kit for Newcastle Disease Virus Virulent and Universal Strains is applicable to detect the NDV Virulent/Universal Strains RNA in bird throat swabs, cloaca swabs, tissue and culture cell samples. The test results are for research purposes only and not for clinical diagnosis.
 
Advantages:
1. GMP, ISO 9001 certificates
2. The product is evaluated by National Avian Influenza Laboratory of China Animal Health and Epidemiology Center.
3. High stability and effectiveness.
4. Customization & Multiple packaging
Availability:


Real-time PCR Detection Kit for Newcastle Disease Virus Virulent and Universal Strains

For Veterinary Use Only

Product NameReal-time PCR Detection Kit for Newcastle Disease Virus Virulent and Universal Strains. 

Package50 Tests

IndicationThe Detection Kit for Newcastle Disease Virus Virulent and Universal Strains is applicable to detect the NDV Virulent/Universal Strains RNA in bird throat swabs, cloaca swabs, tissue and culture cell samples. The test results are for research purposes only and not for clinical diagnosis.

Main components and content

Name

Specification

Quantity

NDV Virulent/Universal Reaction Solution

1000µl/Tube

1

NDV Virulent/Universal Positive Control

250µl/Tube

1

Negative Control

250µl/Tube

1

Storage and Shelf Life

Stored at -20±5℃, Repeated freezing and thawing ≤ 3 times, the shelf life is 12 months.

Test Method

1. Nucleic Acid Extraction

A commercialized RNA/DNA extraction kit can be carried out for nucleic acid extraction, please follow the instruction of the kit.

2. PCR amplification

2.1 Calculate the number of test samples, take n+2 PCR reaction tubes, and add 20µl to of reaction solution to each tube.

2.2 Add 5µl nucleic acid of negative control, positive control and samples into the above PRC reaction tubes respectively, centrifuge at 8000rpm for several seconds, and put them into the Thermal Cycler.

2.3 The reaction conditions are set as follows:

Relevant parameters of the amplifier

System

Total volume: 25µl

Signal collection

NDV Virulent/Universal Fluorescence signals

Virulent - FAM channel collects fluorescence signal

Universal  HEX channel collects fluorescence signal

 

 

PCR reaction conditions

Stage

Condition

Cycle number

UNG process

50℃: 5 minutes

1

Predegeneration

95℃: 30 seconds

1

 

PCR

95℃: 10 seconds

 

40

58℃: 45 seconds

(Set to collect fluorescent signal at the end of this stage)

*Note: Please do not choose ROX correction for ABI series fluorescence quantitative Thermal Cycler, choose None for Quenchers.

 

Interpretation of the results

1. Determination of test kit effectiveness:

1.1 Positive Control: The Ct values of the FAM and HEX channel  32, and the amplification curve has a significant exponential growth phase.

1.2 Negative Control: FAM and HEX channel have no amplification curve, or the amplification curve is a straight line or slightly oblique line.

2. Determination of the results:

Result Judgement

FAM channel

HEX channel

NDV Virulent Strain nucleic acid positive

+

+

NDV Non-Virulent Strain (Vaccine) nucleic acid positive

-

+

NDV nucleic acid negative

-

-

*Note:

If there is an Exponential growth amplification curve and Ct value ≤ 36, it is determined as “+”.

If there is no amplification curve, it is determined as “-”.

If 36 < Ct Value < 40, it is a doubtful sample, and the analysis should be repeated for confirmation.

Precautions

1. The laboratory management shall strictly follow the management specification of the PCR gene amplification laboratory. The laboratory personnel must be trained professionally. The experiment process shall be conducted strictly in different areas (Reagent preparation area, specimen preparation area, amplification and product analysis area). All consumables shall be disposable after sterilization. Special apparatus, equipment and supplies at each stage of the experiment operation shall not be cross-used.

2. Please prepare the biological safety cabinet for reagent and specimen preparation stage. The Lab coat, disposable gloves and pipette shall be carried out during the experiment.

3. Repeated freezing&thawing of reagents shall be avoided as far as possible. Before use, the reagents shall be completely thawed and centrifuged at 8000rpm for several seconds.

4. Please put the pipette used in the specimen preparation area into the container containing disinfectant and discard it with the waste after sterilization.

5. After the experiment, the worktable and pipette shall be treated with 10% hypochlorite or 75% alcohol or ultraviolet lamp.

 

Manufacture

Name: Shandong Xinda Gene Technology Co., Ltd

A subsidiary of the Shandong Sinder Technology Co., Ltd

Address: Building B2, Bandaohuigu Industrial Park, Shungeng Road, Zhucheng City, Shandong Province

Post Code: 262233

Phone: +86 - 0532 5882 0810

 


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Shandong Sinder Technology Co., Ltd is a China animal health joint venture company with SUMITOMO JAPAN that develops, manufactures and markets a broad range of veterinary medicines and services.

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