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This kit is applicable to the nucleic acid detection of SHIV DNA in shrimp muscle tissue The test results are for research purpose only and not for clinical diagnosis.
1. GMP, ISO 9001 certificates
2. High stability and effectiveness.
3. High sensitivity: the minimum detection limit of samples can reach 1.0 copies/ul.
4.High accuracy: good experimental repeatability, amplification efficiency of about 100%
5.Short detection time: one-step multiple detection, only one hour to produce

Product Name: Real-time PCR Detection Kit for Shrimp Hemocyte Iridescent Virus

Package: 50 test/box

Indication: This kit is applicable to the nucleic acid detection of shrimp Decapod iridescent virus DNA in shrimp muscle tissue The test results are for research purpose only and not for clinical diagnosis.

Main components and content:




SHIV Reaction Solution



SHIV Positive Control



Negative Control



Storage and Shelf Life:

Stored at -20±5℃,Repeated freezing and thawing ≤ 3 times, the shelf life is 12 months.

Test Method:

1. Nucleic Acid Extraction

Commercialized DNA extraction kit can be carried out for nucleic acid extraction, please follow the instruction of the kit.

2. PCR amplification

2.1 Calculate the number of test samples, take n+2 PCR reaction tubes, and add 20µl reaction solution to each tube.

2.2 Respectively add 5µl nucleic acid of negative control, positive control and samples into the above PRC reaction tubes, centrifuge at 8000rpm for several seconds, and put them into the fluorescent quantitative PCR.

2.3 The reaction conditions shall be set as:

Parameters of amplification


Total volume is 25µl

Signal collection

SHIV fluorescence signal

FAM channel collects fluorescence signal

Reaction Conditions of PCR




UNG Process

37℃ for 2 minutes



95℃ for 30 seconds



95℃ for 10 seconds


60℃ for 30 seconds

Set to collect the fluorescent signal at the end of this phase

Interpretation of the results:

1. The validity judgement:

1.1 Positive Control: The Ct value of FAM channel ≤ 32, and the amplification curve has a significant exponential growth phase.

1.2 Negative Control: FAM channel has not amplification curve, or the amplification curve is straight line or slight oblique line.

2. The test result judgement:

If the FAM detection channel of the test sample has no amplification curve, it can be determined as SHIV negative.

If the FAM detection channel has an exponential growing amplification curve and the Ct value is 36, it can be determined as SHIV positive.

36<Ct Value<40 samples should be considered as doubtful results, and the analysis should be repeated for confirmation.


1. The laboratory management shall be in strict accordance with the management specification of PCR gene amplification laboratory. The laboratory personnel must be trained professionally. The experiment process shall be conducted strictly in different areas (Reagent preparation area, specimen preparation area, amplification and product analysis area). All consumables shall be disposable after sterilization. Special apparatus, equipment and supplies at each stage of the experiment operation shall not be cross-used.

2. Please prepare the biological safety cabinet for reagent and specimen preparation stage. The Lab coat, disposable gloves and pipettor shall be carried out during the experiment.

3. Repeated freezing&thawing of reagents shall be avoided as far as possible. Before use, the reagents shall be completely thawed and centrifuged at 8000rpm for several seconds.

4. Please put the pipette used in the specimen preparation area into the container containing disinfectant and discard with the waste after sterilization.

5. After the experiment, the worktable and pipettor were treated with 10% hypochlorite or 75% alcohol or ultraviolet lamp.


Name: Shandong Xinda Gene Technology Co., Ltd

A subsidiary of the Shandong Sinder Technology Co., Ltd

Address: Building B2, Bandaohuigu Industrial Park, Shungeng Road, Zhucheng City, Shandong Province

Post Code: 262233

Phone: +86 - 0532 5882 0810


Product Category

Shandong Sinder Technology Co., Ltd is a China animal health joint venture company with SUMITOMO JAPAN that develops, manufactures and markets a broad range of veterinary medicines and services.

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