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LSDV PCR

The Real-time PCR Detection Kit for Bovine Nodular Skin Disease (LSDV) is applicable to detect the Bovine Nodular Skin Disease (LSDV) DNA in blood, tissue and culture cell samples. The test results are for research purpose only and not for clinical diagnosis.
 
Advantages:
1. GMP, ISO 9001 certificates
2. The product is evaluated by National Avian Influenza Laboratory of China Animal Health and Epidemiology Center.
3. High stability and effectiveness.
4. Customization & Multiple packaging
Availability:


Real-time PCR Detection Kit for Lumpy Skin Disease Virus


【Product NameReal-time PCR Detection Kit for Bovine Nodular Skin Disease (LSDV)

【Package50 Tests

【IndicationThe Real-time PCR Detection Kit for Bovine Nodular Skin Disease (LSDV) is applicable to detect the Bovine Nodular Skin Disease (LSDV) DNA in blood, tissue and culture cell samples. The test results are for research purpose only and not for clinical diagnosis.

Main components and content

Name

Specification

Quantity

LSDV   Reaction Solution

1000µl/Tube

1

LSDV   Positive Control

250µl/Tube

1

Negative   Control

250µl/Tube

1

Storage and Shelf Life

Stored at -20±5℃,Repeated freezing and thawing ≤ 3 times, the shelf life is 12 months.

Test Method

1. Nucleic Acid Extraction

A commercialized DNA/RNA extraction kit can be carried out for nucleic acid extraction, please follow the instruction of the kit.

2. PCR amplification (Example of ABI 7500, refer to this operation and manual for ABI 7300 and LC480.)

2.1 Calculate the number of test samples, take n+2 PCR reaction tubes, and add 20µl to of reaction solution to each tube.

2.2 Add 5µl nucleic acid of negative control, positive control and samples into the above PRC reaction tubes respectively, centrifuge at 8000rpm for several seconds, and put them into the fluorescent quantitative PCR amplifier.

2.3 The reaction conditions shall be set as:

Parameters of   amplification

System

Total   volume is 25µl

Signal collection

LSDV   fluorescence signal

FAM channel   collects fluorescence signal

Reaction   Conditions of PCR

Phase

Conditions

Cycles

UNG Process

37℃ for 2   minutes

1

Pre-denaturation

95℃ for 30   seconds

1

PCR

95℃ for 10   seconds

40

60℃ for 30   seconds

Set to collect   the fluorescent signal at the end of this phase

Interpretation of the results

1. The validity judgement:

1.1 Positive Control: The Ct value of FAM channel ≤ 32, and the amplification curve has a significant exponential growth phase.

1.2 Negative Control: FAM channel has not amplification curve, or the amplification curve is straight line or slight oblique line.

2. The test result judgement:

If the FAM detection channel of the test sample has no amplification curve, it can be determined as LSDV negative.

If the FAM detection channel has an exponential growing amplification curve and the Ct value is ≤ 36, it can be determined as LSDV positive.

36<Ct Value<40 samples should be considered as doubtful results, and the analysis should be repeated for confirmation.

Precautions

1. The laboratory management shall strictly follow the management specification of PCR gene amplification laboratory. The laboratory personnel must be trained professionally. The experiment process shall be conducted strictly in different areas (Reagent preparation area, specimen preparation area, amplification and product analysis area). All consumables shall be disposable after sterilization. Special apparatus, equipment and supplies at each stage of the experiment operation shall not be cross-used.

2. Please prepare the biological safety cabinet for reagent and specimen preparation stage. The Lab coat, disposable gloves and pipette shall be carried out during the experiment.

3. Repeated freezing&thawing of reagents shall be avoided as far as possible. Before use, the reagents shall be completely thawed and centrifuged at 8000rpm for several seconds.

4. Please put the pipette used in the specimen preparation area into the container containing disinfectant and discard with the waste after sterilization.

5. After the experiment, the worktable and pipette shall be treated with 10% hypochlorite or 75% alcohol or ultraviolet lamp.

Manufacture

Name: Shandong Xinda Gene Technology Co., Ltd

A subsidiary of the Shandong Sinder Technology Co., Ltd

Address: Building B2, Bandaohuigu Industrial Park, Shungeng Road, Zhucheng City, Shandong Province

Post Code: 262233

Phone: +86 - 0532 5882 0810



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